Mucin gene expression and mouse middle ear epithelium

Kerschner, Joseph E.; Li, Jizhen; Tsushiya, Katsuyuki; Khampang, P.

doi:10.1016/j.ijporl.2010.04.014

« Previous Next »International Journal of Pediatric Otorhinolaryngology
Volume 74, Issue 8 , Pages 864-868, August 2010

Mucin gene expression and mouse middle ear epithelium☆

Joseph E. Kerschner
- Division of Pediatric Otolaryngology, Medical College of Wisconsin, Children's Hospital of Wisconsin, 9000 W. Wisconsin Avenue, Milwaukee, WI 53226, United States
- Department of Otolaryngology and Communication Sciences, Medical College of Wisconsin, Children's Hospital of Wisconsin, 9000 W. Wisconsin Avenue, Milwaukee, WI 53226, United States
- Corresponding author at: Division of Pediatric Otolaryngology, Medical College of Wisconsin, Children's Hospital of Wisconsin, 9000 W. Wisconsin Avenue, Milwaukee, WI 53226, United States. Tel.: +1 414 266 6476; fax: +1 414 266 2693.
Jizhen Li
- Otitis Media Research Center, University of Minnesota, Minneapolis, MN, United States
Katsuyuki Tsushiya
- Otitis Media Research Center, University of Minnesota, Minneapolis, MN, United States
P. Khampang
- Department of Otolaryngology and Communication Sciences, Medical College of Wisconsin, Children's Hospital of Wisconsin, 9000 W. Wisconsin Avenue, Milwaukee, WI 53226, United States

Received 5 April 2010; received in revised form 21 April 2010; accepted 27 April 2010.

Abstract

Objectives

To investigate the expression of recently identified human mucin genes in an in vitro model of cultured mouse middle ear epithelial cells (MMEEC).

Methods

MMEEC were established, RNA was extracted and primers were designed for RT-PCR to assess for expression of mucin genes Muc1, Muc2, Muc3, Muc4, Muc5AC, Muc5B, Muc6, Muc7, Muc8, Muc9, Muc10, Muc11/12, Muc13, Muc15, Muc16, Muc17, Muc18, Muc19 and Muc20 expression.

Results

Mucin genes Muc1, Muc2, Muc3, Muc4, Muc5AC, Muc5B, Muc9, Muc10, Muc13, Muc15, Muc16, Muc18, Muc19 and Muc20 were identified and expressed in MMEEC. The genes Muc6, Muc7, Muc8, Muc11/12 and Muc17 were not identified.

Conclusion

Many of the mucin genes that have been recently identified in human MEE and chinchilla MEE are also expressed in MMEEC. There are differences in expression, however, which may have implications in utilizing various animal models for study of middle ear physiology and pathogenesis; specifically as it relates to mucin gene expression.

Keywords: Mucin gene, Mouse, Middle ear epithelium, Otitis media