Effect of cryopreservation on proliferative features of neural progenitor cells derived from olfactory bulb of embryonic rat

Xue, Tao; Wei, Li; Zheng, Ran-hong; Qiao, Li; Qiu, Jian-hua; Chen, Fu-quan; Lu, Lian-jun; Zha, Ding-jun; Han, Yu; Chen, Jun

doi:10.1016/j.ijporl.2009.03.017

« Previous Next »International Journal of Pediatric Otorhinolaryngology
Volume 73, Issue 7 , Pages 969-973, July 2009

Effect of cryopreservation on proliferative features of neural progenitor cells derived from olfactory bulb of embryonic rat

Tao Xue
- Department of Otolaryngology, Xijing Hospital, The Fourth Military Medical University, Xi’an 710032, China
- These authors contributed equally to this study.
Li Wei
- Department of Obstetrics and Gynecology, Xijing Hospital, The Fourth Military Medical University, Xi’an 710032, China
- These authors contributed equally to this study.
Ran-hong Zheng
- Shaanxi Provincial Health Department, Xi’an 710032, China
- These authors contributed equally to this study.
Li Qiao
- Department of Otolaryngology, Xijing Hospital, The Fourth Military Medical University, Xi’an 710032, China
Jian-hua Qiu
- Department of Otolaryngology, Xijing Hospital, The Fourth Military Medical University, Xi’an 710032, China
- Corresponding author. Tel.: +86 29 84775381; fax: +86 29 83224744.
Fu-quan Chen
- Department of Otolaryngology, Xijing Hospital, The Fourth Military Medical University, Xi’an 710032, China
Lian-jun Lu
- Department of Otolaryngology, Xijing Hospital, The Fourth Military Medical University, Xi’an 710032, China
Ding-jun Zha
- Department of Otolaryngology, Xijing Hospital, The Fourth Military Medical University, Xi’an 710032, China
Yu Han
- Department of Otolaryngology, Xijing Hospital, The Fourth Military Medical University, Xi’an 710032, China
Jun Chen
- Department of Otolaryngology, Xijing Hospital, The Fourth Military Medical University, Xi’an 710032, China

Received 6 January 2009; received in revised form 18 March 2009; accepted 20 March 2009.

Abstract

Objective

Stem cell research offers unique opportunities for developing new medical therapies for devastating diseases and a new way to explore fundamental questions of biology. The use of olfactory bulb neural progenitor cells for transplantation requires efficient recovery methods and cryopreservation procedures. The purpose of this study was to determine cryopreservation techniques for neural progenitor cells derived from olfactory bulb (OB NPCs) of embryonic rat.

Methods

Initially, we compared the survival rates of cryopreserved OB NPCs using three cryoprotectants: dimethyl sulfoxide (DMSO), ethylene glycol (EG) and glycerol with or without 10% FBS. Cells were held at liquid nitrogen (−186°C) for 7 days (“short-term storage”) or 6 months (“long-term storage”). We assessed OB NPCs recovery efficiency after freezing and thawing by viability testing, colony-forming ability and immunocytochemistry under different conditions.

Results

The survival rate of cryopreserved–thawed OB NPCs was estimated by counting colony numbers under a stereomicroscope. No significant difference in survival rate was observed between cryoprotectants.

Conclusions

These observations indicate that cryopreservation of OB NPCs is possible for up to 6 months in optimal conditions without losing proliferation activity.

Keywords: Neural precursor cell, Cryopreservation, Olfactory bulb, Embryonic